ABSTRACT
Objective To analyze the evaluation results of malaria elimination and summarize the experience in Jiujiang City, so as to provide the evidence for formulating the surveillance work plan post-malaria elimination. Methods According to the requirements and arrangements of Jiangxi Eliminating Malaria Action Plan (2010 – 2020), the evaluation of malaria elimination was completed in all 13 counties (cites and districts) of Jiujiang City from 2013 to 2016, and the data were collected and analyzed. Results In Jiujiang City, the last local malaria case was reported in 2008. From 2010 to 2016, there were 20 imported malaria cases reported, including 12 cases of falciparum malaria, 7 cases of vivax malaria, and 1 case of mixed infection. Totally 67 501 fever patients were tested for Plasmodium and 13 cases were positive, with the positive rate of 0.02%. The comprehensive evaluation scores were between 88.75 and 98.15 in all the 13 counties (cities and districts), which meant they all reached the evaluative criteria. Conclusion All 13 counties (cities and districts) of Jiujiang City have passed the city level evaluative criteria of malaria elimination, and the key work should be the prevention and surveillance of imported malaria in the future.
ABSTRACT
Objective To analyze the evaluation results of malaria elimination and summarize the experience in Jiujiang City, so as to provide the evidence for formulating the surveillance work plan post-malaria elimination. Methods According to the requirements and arrangements of Jiangxi Eliminating Malaria Action Plan (2010 – 2020), the evaluation of malaria elimination was completed in all 13 counties (cites and districts) of Jiujiang City from 2013 to 2016, and the data were collected and analyzed. Results In Jiujiang City, the last local malaria case was reported in 2008. From 2010 to 2016, there were 20 imported malaria cases reported, including 12 cases of falciparum malaria, 7 cases of vivax malaria, and 1 case of mixed infection. Totally 67 501 fever patients were tested for Plasmodium and 13 cases were positive, with the positive rate of 0.02%. The comprehensive evaluation scores were between 88.75 and 98.15 in all the 13 counties (cities and districts), which meant they all reached the evaluative criteria. Conclusion All 13 counties (cities and districts) of Jiujiang City have passed the city level evaluative criteria of malaria elimination, and the key work should be the prevention and surveillance of imported malaria in the future.
ABSTRACT
Objective: A method of reversed phase high performance liquid chromatogram-light scattering detector (RP-HPLC-ELSD) was developed to separate and prepare a large number of 25-OH-PPD epimeric mixture, in order to explore the best separation conditions between method of continuous sampling interval and off-line preparation method. Methods: The separation consequence of the method of continuous sampling interval and off-line preparation method were investigated respectively, under different proportion of mobile phase and concentration of sample volume, the optimum preparation method was screened by comparing the efficiency and transfer rate. Results: The suitable operation conditions of the two methods and the result of the preparation were obtained: method of continuous sampling interval as follow: the mobile phase is methanol and water (83:17), and the volume flow and injection volume are 20 mg/mL and 1 mL, respectively, while the flow rate is 20 mL/min; Under above conditions the preparation efficiency of 20(S)-25-OH-PPD and 20(R)-25-OH-PPD is 18.01 and 35.36 mg/h, respectively; Off-line preparation method, the mobile phase is methanol and water (81:19), and the volume flow and injection volume is 200 mg/mL and 2.5 mL, while the flow rate is 20 mL/min, The preparation efficiency of 20(S)-25-OH-PPD and 20(R)-25-OH-PPD is 50.55 and 51.93 mg/h, respectively. Conclusion: Preparation efficiency of off-line preparation method is higher than that of the method of continuous sampling interval; This method is convenient and reliable and has large amount of 20(S)-25-OH-PPD and 20(R)-25-OH-PPD, which can establish a good foundation for the separation and preparation of 25-OH-PPD isomer.